Recovery of Soil Microbial Community Structure after Fire in a Sagebrush-grassland Ecosystem

Recovery of the soil microbial community after fire in a sagebrush-grassland ecosystem was examined using a chronosequence of four sites ranging in time since fire from 3–39 years. The successional stage communities examined included Recent Burn (3 years since fire, ysf), Establishment (7 ysf), Expansion (21 ysf), and Mature (39 ysf). Aboveground standing plant biomass increased with time since disturbance to the Mature stage where sagebrush became dominant over herbaceous species. Phospholipid fatty acid (PLFA) analysis was used to characterize the microbial community structure. Soil microbial community productivity generally appeared to be similar to the Mature site soil (39 ysf) within 7 years of fire. Diversity of PLFAs detected in soils, at both depths, increased from a low value of 29 at the Recent site to a high of 37 at the Establishment site and then decreased again to 31 at the Mature stage site. Canonical variates analysis indicated important disparities in microbial community structure at the four sites. Greatest disparities were observed in microbial community structure between the Recent and Establishment stages but greater similarity between the Recent stage and the sagebrush dominated Mature stage. This study emphasizes both short-term and long-term changes in the belowground community and suggests that soil microbial communities are highly resilient to disturbances after prescribed fire. Copyright # 2010 John Wiley & Sons, Ltd. key words: canonical variates analysis; fire; microbial diversity; phospholipid fatty acid analysis; sagebrush-grassland; USA